Ovulation Test Strips Instructions for Use

    Dip the strip into the urine for 3-5 seconds

    Lay the strip flat 

    Read results in 5 minutes

    Positive (LH Surge): If two color lines are visible and the test line is equal to or darker than the control line. A positive result indicates the presence of a high amount of Luteinizing Hormone or LH surge.

    Negative (No LH Surge): Only one line is visible or the test line is lighter than the control line.



    Determine the length of your menstrual cycle before the test (The length of the menstrual cycle is the duration from your first menstrual bleeding day to the day before the next bleeding begins). Please refer to the chart below to determine when you should start testing. If you do not know your cycle length, you may begin the test 11 days after your first period since the average cycle length is 28 days.

    Example: If your cycle length is normally 26 days,the chart indicates testing should begin on day 10.


    Ovulation is the release of a mature egg from the ovary. It signals the beginning of your fertile period. LH (Luteinizing Hormone) is one of the hormones produced by the pituitary gland. It is secreted at very low levels throughout your menstrual cycle. Once a developing egg follicle reaches a certain size, LH secretion surges to high levels. This hormone surge is what triggers ovulation about 24 to 48 hours later. Your LH surge is important because it initiates the beginning of ovulation.  A positive result indicates the presence of a high amount of Luteinizing Hormone or LH surge.


    When to collect urine for the test? Any urine specimen is appropriate for  ovulation testing. The ideal time for ovulation test is in the afternoon, though testing may take place from 10am to early evening.

    How long should I continue to perform the test?
    At least 5 days or until the LH surge has been detected.

    Once I see a positive result, when is the best time to have intercourse? 
    Ovulation is likely to occur within 24-48 hours. This is the most fertile time. Sexual intercourse is advised within this time frame.

    I have received a positive result and had intercourse during these fertile days. I have not become pregnant. What shall I do?
    Many factors can affect your ability to become pregnant. Often you may need to use the test kit for 3-4 months. You and your partner should consult your physician if pregnancy is not achieved after 3-4 months.

    Will the amount of liquid I drink affect the result? 
    We suggest that you limit your fluid intake for about two hours before you collect your urine. Because heavy intake of fluids prior to testing will dilute the hormone in your urine.

    Can test results be interpreted after more than 5 minutes?
    No. Test results must be read at 5 minutes. Though a positive result should not change for several days, a negative result may change to a false positive within minutes after the end of the testing period, which would not be an accurate reading. It is always best to read the results at the 5 minute testing period and then discard the test to avoid confusion.

    Can any medication or medical condition affect the result? Certain medical conditions and medications can adversely affect the performance of the test: for example if you are actually pregnant, have recently been pregnant, have reached menopause or have polycystic ovarian syndrome you may get a misleading result. This may also be true if you are taking fertility drugs containing Luteinizing Hormone or Human Chorionic Gonadotrophin (such as  Pregnyl® and Profasi®). Clomiphene citrate (Clomid®) does not affect the tests, but may affect the length of your cycle and, therefore, when you should be testing.